PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF Tricalysia chevalieri K. KRAUSE LEAVES EXTRACT
Keywords:
Tricalysia chevalieri, Antimicrobial susceptibility, Phytochemical composition, Bacterial isolates.Abstract
An assessment of phytochemical composition and antimicrobial activity of methanol leaf
extract of Tricalysia chevalieri was evaluated for the presence of phytochemicals. The bacterial
pathogens used were subjected to biochemical tests for proper identification and antibacterial
susceptibility of the crude extracts against clinical isolates of Escherichia coli and Klebsiella
pneumoneae were determined at varying concentrations 200mg/ml 100mg/ml 50mg/ml
25mg/ml using agar well diffusion method. The MIC and MBC of the crude extracts were also
determined. The result of Phytochemical screening revealed the presence of Saponins, Tannins,
Steroids, Glycosides, Phenols and Flavonoids in the crude extracts. The methanol leaf extract
exhibited good activity against the test organisms. The Minimum Inhibitory Concentration
(MIC) and Minimum Bactericidal Concentration (MBC) of the leaf extract ranges from 50-
100mg/ml for Escherichia coli and Klebsiella pneumoneae 25-200mg/ml respectively. The
extract showed significant inhibition against all the test organisms.hence bactericidal in its
effects. This study concludes that the methanol leaf extract of Tricalysia chevalieri contains
bioactive components that have broad spectrum antimicrobial properties that can be used as a
good source of drugs to cure Dysentery and Pneumonia.
