IN VITRO ANTHELMINTIC ACTIVITY OF AQUEOUS AND METHANOLIC EXTRACTS OF Guiera senegalensis AGAINST FOUR SPECIES OF TRICHOSTRONGYLID NEMATODES

Abstract

Gastrointestinal nematodes increasing resistance to synthetic drugs and the side effects of the
drugs as it interferes with the quality of animal products call for a search of alternative
anthelmintic from plants. This research evaluates the anthelmintic potential of methanol and
aqueous extracts of G. senegalensis against the migration of H. contortus, T. colubriformis, O.
circumcincta and Oesophagostomum spp. Each of the species was incubated in 0.2, 0.4, 0.6,
0.8, 1.0, and 2.0 mg/ml of aqueous and methanol extracts of G. senegalensis and later
transferred to migratory plates. Larvae in the positive control were treated with 0.02 μg/ml of
ivermectin while those in the negative control were treated with M9. Both methanol and
aqueous extracts exhibited good larvicidal activities against the migration of all the species of
the nematodes. However, the efficacy of methanol extract was higher than aqueous extract in
every tested species (P < 0.05). The most sensitive of all the species to the extracts was H.
contortus which recorded the lowest IC50 values of 0.631 and 0.350 mg/ml for aqueous and
methanol extract respectively. The less sensitive of all the species was Oesophagostomum spp
as it recorded the highest IC50 values of 0.840 and 0.535 mg/ml for aqueous and methanol
extracts respectively. Ivermectin was more efficient that the plant extracts.