ISOLATION AND STRUCTURE ELUCIDATION OF IPOLAMIIDE FROM THE STEM BARK OF STACHYTARPHETA ANGUSTIFOLIA MILL VAHL (VERBENACEAE)

Abstract

This research work is aimed at isolating and structural elucidation of the compound from the stem
bark extract of S. angustifolia using standard phytochemical and spectroscopic techniques. The
grounded powdered material of the stem bark was extracted and partitioned using various solvent
of different polarity. Column chromatography, n-BuoH soluble fraction of the ethanol extract of
stem bark S. angustifolia was run using silica gel and subsequently pooled fractions from various
portions were purified using sephadex LH20 to obtain compound 1. Pure isolate of compound 1
was subjected to analysis using FTIR, 1and 2D NMR. Compound 1 was determined as an Iridoid
glucoside Cyclopenta [C] pyran-4-Carboxylic acid, 1-(β-D glucopyranosyloxy)-1, 4A, 5,6,7,7A
Hexahydro-4A, 7- dihydroxy-7- methyl-, Methyl Ester, (1S, 4AR,7S, 7AR) (Ipolamiide), MP 218-
220oC, MW C17H27O12, [M]+424(EIMS) on the basis of spectral analysis and Comparism with
reference data.